The stems of Dendrobium officinale

The stems of Dendrobium officinale Kimura et Migo, named Tie-pi-shi-hu,
is one of the most endangered and precious species in China. Because of its various
pharmacodynamic effects, D. officinale is widely recognized as a high-quality health food
in China and other countries in south and south-east Asia. With the rising interest of
D. officinale, its products have a high price due to a limited supply. This high price has led
to the proliferation of adulterants in the market. To ensure the safe use of D. officinale, a
fast and convenient method combining normal and fluorescence microscopy was applied
in the present study to distinguish D. officinale from three commonly used adulterants
including Zi-pi-shi-hu (D. devonianum), Shui-cao-shi-hu (D. aphyllum), Guang-jie-shi-hu
(D. gratiosissimum). The result demonstrated that D. officinale could be identified by the
characteristic “two hat-shaped” vascular bundle sheath observed under the fluorescence
microscopy and the distribution of raphides under normal light microscopy. The other three
adulterants could be discriminated by the vascular bundle differences and the distribution of
raphides under normal light microscopy. This work indicated that combination of normal light
and fluorescence microscopy is a fast and efficient technique to scientifically distinguish
D. officinale from the commonly confused species.

Results (English) 2:

The Stems of Dendrobium officinale Kimura et Migo, named Tie-PI-shi-hu,
is one of the MOST and precious Endangered Species in China. Because of ITS Various
pharmacodynamic Effects, D. officinale is Widely Recognized as a High-quality Health Food
in China and other Countries in south and south-east Asia. With the Rising Interest of
D. officinale, ITS Products have a High price Due to a Limited supply. This High price has led
to the Proliferation of adulterants in the Market. To Ensure the Safe Use of D. officinale, a
fast and Convenient Combining normal and Fluorescence Microscopy method was applied
in the Present study to distinguish from D. officinale Three Commonly Used adulterants
Including Zi-PI-shi-hu (D. devonianum), Shui- cao-shi-hu (D. aphyllum), Guang-jie-shi-hu
(D. gratiosissimum). The result Demonstrated That D. officinale could be IDENTIFIED by the
Characteristic "Two Hat-shaped" Vascular bundle sheath the observed under the Fluorescence
Microscopy and the Distribution of raphides under normal light Microscopy. The other Three
adulterants could be discriminated by the Vascular bundle Differences and the Distribution of
raphides Microscopy under normal light. This Work Indicated That Combination of normal light
and Fluorescence Microscopy Technique is a fast and Efficient to scientifically distinguish
from the D. officinale Species Commonly confused.

Results (English) 3:

Stems of Dendrobium officinale Kimura et Migo, Tie-pi-shi-hu named, one of the most endangered and precious species in China. Because of various
pharmacodynamic effects
is its D. officinale recognized widely is as a high-quality health food
in China and countries other South in south-east and With Asia. rising interest of
D. the officinale The,Products have a high price due to a limited supply. This price high led
to has proliferation the adulterants of the in To ensure the safe use market. D. of officinale and a
fast convenient method combining normal and fluorescence microscopy was applied
in the present study to distinguish D. officinale from three commonly used adulterants
including Zi-pi-shi-hu its (devonianum D.),Shui-cao-shi-hu (aphyllum D.), Guang-jie-shi-hu
(gratiosissimum The). Result demonstrated that D. officinale could be by identified two the
characteristic hat-shaped vascular bundle sheath observed under the fluorescence
microscopy and the distribution of raphides under normal light microscopy. The other three
D.Could be discriminated by the vascular bundle differences and the distribution of
raphides under normal light microscopy. This and indicated work combination that normal of fast light
and fluorescence microscopy is a